WESTERN BLOT TROUBLESHOOTING SMEAR

Aug 1, 11
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  • No signal in southern blot - troubleshooting (reply: 2); Help! Campylobacter Southern Blotting problems - can't get any signal from . how does depurination helps in Southern blotting - (reply: 1); smear band in southern hybridization - (reply: 2) . labeling on southern, northern and western blot - (reply: 1) .
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  • Western Blot - Any one ever use serum as a primary antibody? (reply: 2) . .. Western Blot Troubleshooting - Marker fading and other issues (reply: 7) . .. 38kda protein appearing as smear on western - (reply: 5); Western Blotting .
  • (Western blots can be developed with the FIVEphoton Biochemicals Chemiluminescence . The transcription factor resolves as a smear in the nuclear fraction. . .. Troubleshooting. a. Cells do not appear to lyse with the recommended .
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  • . PCR, cloning, DNA sequencing, or Southern blotting for further characterization. . or to indistinguishable smears representing multiple unresolved components. . . SDS-PAGE · Two-dimensional gel electrophoresis · Western blotting . Troubleshooting DNA agarose gel electrophoresis. Focus 19:3 p.66 (1997). .
  • Troubleshooting guides for DNA digestion, PCR, RT-PCR, molecular cloning . This will result in a band or smear above the expected band (see picture below). . .. For Coomassie based stains and Western blot applications use 0.5-5 µg of .
  • Non-specific bands with secondary Antibody on Western Blotting - (reply: 1) . Maxiprep troubleshooting - There are 3 bands in CsCl gradient after . Optimum annealing temperature were fail with no band and just smearing - Optimum .
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  • Carbonate-based blotting transfer solution recipie? troubleshooting . The troublesome thing is that there is always smear on the gel according to the p . .
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  • 3 May 2010 – Blood smear preparation and staining. Buffers (see Solutions below) . . troubleshooting guide (PDF file, 66k) . Histone Western blotting .
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  • 5 posts - 3 authors - Last post: 12 Dec 2008Trouble-shooting Tris-Tricine SDS-PAGE - (Dec/11/2008 ) . The smearing only gets worse the longer the gel runs and sometimes it is so bad .
  • Western blot troubleshooting tips. . Western blot troubleshooting tips. Troubleshooting guide to western blot. View a pdf of this page PDF. Contents .
  • 2 posts - 1 authorHelp troubleshoot SDS gel - smear down well - (May/01/2008 ). Hello, I can .
  • Novex® 20% TBE Gel: A high molecular weight band and a smear . If the band representing d-siRNA is weak or if you do not see a band, see Troubleshooting. . .. If you are performing Western blot analysis, you may also use the Anti V5 .
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  • large protein transfer efficiency for western blot Troubleshooting . . Sometimes we get a discreet band, but most of the time its a smear. .
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  • Western blotting protocols, methods and techniques, also includes troubleshooting guides. . large protein transfer efficiency for western blot troubleshooting . . random specks andtiny smears all over the membrane. Western blot with .
  • Technical FAQs · Get technical help · Protocols & troubleshooting · Buying .
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  • Adipogen offers online General Tips & Troubleshooting for IHC/ICC/IF application, . Though less sensitive quantitatively than immunoassays such as Western blotting or ELISA, . deposited from suspension, or taken from a smear. .
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  • 25 Jul 2006 – Troubleshooting. Poor BN-PAGE Stacking Gel Wells; Poor Sample Entry into Gel . Western Blotting Failure; "Smearing" During BN-PAGE Separation . Analysis of the BCR by BN-PAGE Followed by Western Blotting .
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  • Bioassay · IHC · Western Blot · Multiplex Assays/Arrays · Apoptosis Assays . Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC .
  • 12 May 2011 – Tubulin loading control Western blot is an important control experiment to know your reagents . . Smearing effect for the band of interest .
  • Help troubleshoot SDS gel - smear down well - (reply: 2) . Just a smear of proteins after ponceau red - Western Blot (reply: 4) .
  • 3 posts - 1 author - Last post: 6 Nov 2008Hi All, I've run my western several times, but I keep having the same problem. . The smear is roughly where I would expect to see the protein, . Western Blot Background Troubleshooting, molecule2005, Western Blot .
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  • Bioassay · IHC · Western Blot · Multiplex Assays/Arrays . Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC . Carefully remove the coverslips from the wells and blot to remove any . Note: Initial IHC/ICC studies often require further optimization and/or additional troubleshooting steps. .
  • . with ethidium bromide, appears as a smear below 100 bp on an agarose gel. . . The resulting membrane was subjected to Western blot analysis using the same . . Mahadevan for valuable advice in troubleshooting the ChIP protocol. .
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  • A molecular biology portal for molecular protocols and methods, listings for .
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  • 1 Jun 2011 – 2 posts - 1 authorHelp troubleshoot SDS gel - smear down well . For more western blot tips, see the western blot Troubleshooting section. .
  • 15 posts - 7 authors - Last post: 28 Marwestern blot troubleshooting. Use this category for questions regarding . One of the antibodies showed smear on the whole blot with white .
  • 2 posts - 1 author - Last post: 5 Aug 2009Ab smear obscures signal on IP-western - Protocols and Methods Forum .
  • Troubleshooting. 1. Cells detached from cover slip. . .. (Western blots can .